Comparative Analysis of Adult Mesenchymal Stem Cells

September 29, 2020

Comparative Analysis of Adult Mesenchymal Stem Cells Derived from Adipose, Bone-Marrow, Placenta, and Umbilical Cord Tissue

Mesenchymal stromal/stem cells (MSCs) have the potential to repair and regenerate damaged tissues, making them attractive candidates for cell-based therapies. Expanded and well-characterized MSCs have application in regenerative medicine and have been used in several clinical trials including treatment for osteoarthritis and other conditions. Here, we provide results of a comparative study of purified and expanded MSCs from adipose, bone-marrow, placenta, and umbilical cord involving determination of phenotype by flow cytometry analysis, cellular potency by quantitative assessment of mitochondrial function and immunosuppression, and cellular function by quantitative assessment of cell migration and proliferation. Our results show comparable phenotypic profiles, morphology, expansion in cell culture and adipogenic, osteogenic and chondrogenic differentiation. Potency measures of mitochondrial/immunosuppressive capacity and additional cellular function assays show differences suggesting biological advantages of umbilical MSCs. 

Continue reading




Also in White Papers

AlloEx®: A Comprehensive Analysis of Secretomes for Advanced Dermatological Applications

June 06, 2024 0 Comments

The scientific community is increasingly delving into the nuanced realms of secretomes, due to their nature of cellular communication and potential applications in regenerative medicine. These microscopic entities, which are potential essential players in intercellular signaling and tissue regeneration, have received significant attention for their possible therapeutic potential. 

Read More

Exosomes Secreted by the Cocultures of Normal and Oxygen–Glucose‐Deprived Stem Cells Improve Post‐stroke Outcome

September 14, 2022 0 Comments

Emerging stroke literature suggests that treatment of experimentally induced stroke with stem cells offered post-stroke neuroprotection via exosomes produced by these cells. Treatment with exosomes has great potential to overcome the limita- tions associated with cell-based therapies. 

Read More

Mesenchymal Stem Cell Treatment Prevents Post-Stroke Dysregulation of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases

September 14, 2022 0 Comments

Stem cell treatment is one of the potential treatment options for ischemic stroke. We recently demonstrated a protective effect of human umbilical cord blood-derived mesenchymal stem cells (HUCB-MSCs) in a rat model of ischemic stroke. 

Read More